A Laboratory is in need of a Research Assistant II with experience working with genetically engineered mice and experience maintaining records for experiments.
KEY RESPONSIBILITIES:
Under supervision, identify, design, plan, and independently conduct assays & experiments, & assist in interpreting results.
Specific Examples: (A) Transfer cells or manipulate mice, then immunize mice, administer pharmaceutical test agent per protocol, collect sera, score mice for allergic lung disease; ultimately, recover sera, cells and tissues from the experimental subjects, and analyze these sera, tissues, and cells. (B) Prepare purified cells from mice, stimulate and grow in tissue culture, analyze cells and their products. (C) Interface with histology core and with collaborating partners
Ensure preparedness of project and laboratory for experiments and procedures.
Specific Examples: ( A) organize, breed, and determine genotypes of genetically engineered mice to be used in experiments. (B) Arrange for calibration and safety checks on Biological Safety Cabinets required for cell cultures in experiments. (C) Monitor supplies of compressed carbon dioxide and liquid nitrogen and arrange resupply in timely manner needed for the cell culture incubators and for storage of cell lines and samples. (D) Take charge of accurate and timely proposals of ordering via eProcurement, and then tracking receipt and proper distribution or storage of orders received
Provide appropriate figures with data, progress reports, and other written as well as oral documentation of experiments.
Specific Examples: (A) Quantify, tabulate and process ELISA and flow cytometry assay data measuring effects of pharmaceutical agent(s) on allergic lung inflammation. (B) Organize complex sets of data from flow cytometry, cell counts, and ELISA into Powerpoint slides. (C) Prepare and engage in individual (with Research Asst. Professor as well as with PI/lab head) as well as team reviews of written and verbal reports [several times a week with Res. Asst. Prof.; every two weeks for PI meetings; weekly presentation of ongoing flow of data at overall group meeting
Prepare, operate, and troubleshoot equipment in experimental procedures.
Specific Examples: (A) set up flow cytometer and adjust settings for use in analyses of fluorescent staining of cell suspensions in experiments. (B) Operate, and line up maintenance or repairs as needed, various centrifuges [desktop microcentrifuges; table-top and high-speed floor models; preparative ultracentrifuges.
PREFERRED EDUCATION:
Bachelor's or Masters Degree and 2 years experience.
Vanderbilt University Medical Center is home to Vanderbilt University Hospital, The Monroe Carell Jr. Children’s Hospital at Vanderbilt, the Vanderbilt Psychiatric Hospital and the Vanderbilt Stallworth Rehabilitation Hospital. These hospitals experienced more than 61,000 inpatient admissions during fiscal year 2015. Vanderbilt’s adult and pediatric clinics treated nearly 2 million patients during this same period.Vanderbilt University Hospital and the Monroe Carell Jr. Children’s Hospital at Vanderbilt are recognized again this year by U.S. News & World Report’s Best Hospitals as among the nation’s best with 18 nationally ranked specialties. Vanderbilt University Medical Center is world renowned because of the innovation, work ethic and collegiality of its employees. From our health care advances to our compassionate care, Vanderbilt owes its accomplishments and reputation to staff and faculty who bring skill and drive and innovation to the medical center day after day. World-leading academic departments and comprehensive centers of excellence pursue scientific discoveries and transformational educational and clinical advances across the entire spectrum of health and disease.As th...e largest employer in middle Tennessee, we welcome those who are interested in ongoing development in a caring, culturally sensitive and professional atmosphere. Most of us spend so much of our lives at work, we want to be part of maintaining a workplace in which people support one another and encourage reaching for excellence. Many high-achieving employees stay at Vanderbilt because of the professional growth they experience and because of their appreciation of Vanderbilt’s benefits, public events and discussions, athletic opportunities, beautiful setting and, above all, sense of community and purpose.Vanderbilt and its employees share a set of mutual expectations that have been created with productivity, legality, fairness and safety always in mind. We believe that our investment in training and compensating employees multiplies in value when we enable individuals to deliver their best performance for the benefit of us all.
